Binding curves of neutrophil L-selectin interacting with (s)PSGL-1.

<p>Adhesion frequency vs. contact duration plots of neutrophils constitutively expressing native L-selectin interacting with RBCs coated with matched densities of sPSGL-1 (square) or PSGL-1 (triangle) at 59 µm⁻² (A) or 25 µm⁻² (B). Control for nonspecific adhesion was measured using RBCs coated with the capture antibody PL2 without incubation with (s)PSGL-1. The differences in the first three data points in (A) between sPSGL-1 and PSGL-1 interacting with L-selectin are not significant as assessed by the Student-t test (<i>p</i> = 0.32, 0.15, and 0.75).