Cytosolic pH changes in palisade mesophyll, spongy mesophyll, and epidermal cells of saintpaulia leaf.

<p>(A) Palisade mesophyll, spongy mesophyll, and upper and lower epidermal cells stained with BCECF-AM. BCECF-AM (green) and chlorophyll fluorescence (magenta) were observed before, immediately after (0 min), and 10 min after the temperature was decreased from 25°C to 5°C and kept at 5°C for 1 min. Scale bar = 40 µm. (B) Relative changes of BCECF-AM fluorescence intensity after a temperature decrease. The points and associated bars indicate mean intensity and standard error (n = 3).