Motility characteristics of spermatozoa detaching from uterine epithelium in non-capacitating and capacitating conditions during prolonged culture.

<p>Sperm samples diluted with M199 were incubated with proestrus uterine epithelial explants for either (a) 4 hours, (b) 24 hours, (c) 48 hours, (d) 96 hours before transfer to new medium which was (a) non-capacitating control (M199), (b) solublised zona pellucida in CCM (CCM+SZP). Data are mean number (± S.E.M.) of non-motile spermatozoa per plate (NNM) and mean percentage (± S.E.M.) motility characteristics (FFPM; fast forward progressive motility, TM; transitional motility, HM; hyperactive motility) of detaching spermatozoa over 1.0–2.0 hours culture after change of the media. Comparisons are individual motility characteristics between treatments; means with a letter in common are not significantly different p<0.05.