Comparison of Kd of purified prolamins for <i>ELISA Systems</i> and <i>RidaScreen</i> sandwich assays.

<p>The data were replotted as linear Michaelis-Menton plots, and the Kd values determined from the curves of best fit (GraphPAD Prism 5.04). Means±SE of two experiments are shown.</p>a<p>The suffix _T refers to total hordein; _C refers to FPLC purified C-hordeins; _γ refers to FPLC purified γ-hordeins; _B refers to FPLC purified B-hordeins. Commercial gliadin standards used are denoted as Gliadin.</p>b<p>The Kd values were log₁₀ transformed to correct for heterogeneity of variance, and one-way ANOVA conducted on the data for each assay type. In both analyses the p-values for the F-tests for hordein were <0.001. Within each assay type log₁₀(Kd) values with the same symbol were not significantly different at the 5% experiment-wise error rate (GenStat). A two-way ANOVA conducted on data for both assay types (excluding ULG 2.0_T and RisØ 1508_T hordeins) indicated a strong interaction between hordein and assay type (p<0.001), largely due to a lack of a significant difference between the gliadin response for each assay type (p = 0.734).