ELISA immunoreactivity of mAbs to native and denatured PrP^Sc.

<p>Gdn (+) denotes the 4M GdnSCN-treated wells (denatured PrP^Sc). Gdn (−) wells were treated with sterilized water instead of 4M GdnSCN (native PrP^Sc).</p><p>MAbs 6A12 and 8D5 were generated in this study. MAb 6H4 (Prionics) is an anti-PrP antibody that recognizes PrP amino acid residues 143–154.</p>a<p>Data represent mean optical density (O.D.) ± standard deviation in triplicate experiments.</p>*<p>Asterisks indicate a statistically significant difference in mAb immunoreactivity between denatured PrP^Sc and native PrP^Sc (<i>P</i><0.001).