Characterization of bacterial community present in retail poultry products.

<p>A) PCR results for amplification from total nucleic acids (lane 1), DNase treated reverse-transcriptase negative control (lane 2), and reverse-transcribed cDNA (lane 3). PCR reactions were performed with the broad-range primers 104F/530R as described in the text. Figure shows pooled products obtained from six retail packages of uncooked chicken parts; MW marker is NEB 100 bp ladder. B) Relative abundance of sequence types recovered from total nucleic acids (upper graph) as compared to sequence types present in the presumed potentially metabolically active fraction of the community (lower graph) classified to the genus level. Genera marked in bold (21 of 28) were designated potentially metabolically active as determined by sequence detection in the cDNA fraction. Genera marked with vertical arrows were also present in the carcass rinse or weep samples of the longitudinal data set. Genera marked with black circles were present in the fecal or litter samples of the longitudinal data set. Note break in Y axis for <i>Pseudomonas</i> which comprised 98% of both fractions. C) Comparison of species-level classification of sequences for <i>Pseudomonas</i> in DNA (upper graph) and cDNA (lower graph) fractions.