Deletion analysis of the sequence upstream of the coat protein gene and detection of viral RNAs in protoplasts.

<p>(A) The wtTNV-A^C (pMTC 27) construct is shown on top, and the two regions (nt 2312–2610 and nt 2553–2610) upstream of the CP gene that were subjected to deletion are expanded below, and the deleted nucleotides are indicated by dashed lines. Viral RNA accumulation in tobacco BY-2 protoplasts transfected by RNAs containing deletions within the nt 2312–2610 region (B) or the nt 2553–2610 region (C) were assessed by Northern blots at 18 hpt with the same probe used in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057938#pone-0057938-g001" target="_blank">Fig. 1</a>. Illustration of the viral RNAs and rRNA species are indicated on the left side of the gel photographs in the panels C and D.