Effects of nab-paclitaxel on cell cycle progression, expression of phospho-stathmin and mitotic cell death in vitro.

<p>(A) SNU16 cells were cultured for 24 hours and then treated with 100 nM nab-paclitaxel for 12 hours. Cell cycle analysis was performed by flow cytometry. Results shown are representative of two independent experiments. (B–D) Expression of phospho-stathmin and mitotic cell death was evaluated in SNU16 (B), NCI -N87 (C), and AGS (C) cells. Cultured cells were treated with nab-paclitaxel (10 μM) and showed increased mitotic arrest configuration and phospho-stathmin expression by immunocytochemical staining. Mitotic arrests were detected in the presence of phospho-stathmin expression. (D) A sub-confluent monolayer of human gastric cancer cells AGS, NCI-N87 and SNU16 was treated with nab-paclitaxel (10 μM) for 3, 6 and 9 hours and analyzed by immunoblotting for phospho-stathmin and total stathmin. (E) Human gastric cancer cell were treated with nab-paclitaxel (10 μM), oxaliplatin (10 μM), and epirubicin (10 μM) for 16 hours and analyzed for cleaved PARP-1 and caspase-3. Data are representative of two independent experiments with similar results.