Effects of nab-paclitaxel treatment on cell proliferative and apoptotic activity in vivo.

<p>SCID mice were subcutaneously injected SNU16 cells (20×10⁶) and treated with nab-paclitaxel for 2 weeks. (A) Intratumoral proliferation was measured by immunostaining tissue sections with Ki67 nuclear antigen followed by fluorescent microscope photography. Ki67-positive cells were counted in five high power fields per sample. Fold change in proliferative index was standardized to controls (set to 1) and other samples are compared relative to this sample. (B) Intratumoral apoptosis was measured by staining tumor tissue section with the TUNEL procedure and subsequent fluorescent microscope photography. TUNEL-positive apoptotic cells were counted in five high power fields per sample. For both immunostaining experiments, each group had at least three samples counted and the data are expressed as the mean ± standard deviation. Symbol * represents significant difference compared to vehicle group (p<0.05).