Capsaicin and capsazepine effects on heat shock protein expression in HEK293e, MLE-12, MCF-7 and HT-29 cells.

<p>Representative immunoblot analysis of Hsp70, Hsp90 and Hsp27 abundances. Hsp70, Hsp90 and Hsp27 were detected in HEK293e (Panel A), MLE-12 cells (Panel B), HT-29 (Panel C) and MCF-7 (Panel D). Cells in culture were incubated with 0.01% DMSO, 32 µM of capsaicin (Caps) for 1 hr, 32 µM of capsaicin (Caps) for 1 hr with the addition of 100 µM capsazepine (CPZ) or 100 µM capsazepine alone at 37°C. Cells were treated with either heat shock at 42°C for 3 hrs, heat shock at 42°C for 3 hrs with the addition of 100 µM capsazepine. Cells were treated with 32 µM of capsaicin with the addition of 5 mM EGTA, 5 mM EGTA alone at 37°C or heat shock at 42°C for 3 hrs with the addition of 5 mM EGTA. 15 µg of total protein/lane were separated on 9% SDS-PAGE gel.