Inducing FN1BP1 expression in Hep3B cells reduced cell proliferation and colony formation.

<p>After screening in the medium containing hygromycin for more than 8 wk, a total of 33 independent Hyg-resistant cell lines were obtained from the Hep3B-Tet-on cells transfected with pTRE2hyg-FN1BP1. (A) The 11th sense clone of Hep3B Tet-On-FN1BP1 (marked as Hep3B-Tet-On-FN1BP1/S11) showed a positive band identified by either anti-HA tag antibody (a) or anti-FN1BP1 antibody (b) when the cells were induced by Dox. S10 and S18 represent the 10th and 18th sense clones of Hep3B Tet-On-FN1BP1. AS3 represents the third antisense clone of Hep3B Tet-On-FN1BP1, respectively. (B) The cell proliferation activity curve of 6 days shows that expression of FN1BP1 suppressed the Hep3B cell proliferation activity significantly from day 3 to day 6. The data are represented as mean ± SD, * P<0.05 vs non-induced S11 cells, n = 4. (C) The data indicate that the expression of FN1BP1 induced by Dox distinctly decreased the cell colony formation compared to non-induced Hep3B cells, *P<0.05, n = 4. The upper panel shows the representative figure, while the statistical result is shown in the lower panel.