Isolation of hordein fractions by FPLC.

<p>A: The eluant A280 from a 1 mL injection of Urea/DTT extract from cv Sloop, Risø 1508, Risø 56 or ULG 2.0 is shown with individual curves offset for clarity. The identity of peaks containing oxidised DTT (DTT_ox), B- (B-Hor), C- (C-Hor), and γ (γ-Hor)-hordeins of cv Sloop are shown. The eluate from 20 mL to 80 mL was pooled and lyophilised for total hordeins from each line. B & C: Fractions enriched for C-hordeins from cv Sloop (B, peak 3) and Risø 56 (C, peak 8); and γ-hordeins from Risø 56 (C, peaks at 9) were isolated by pooling the indicated eluate (–). Peak 2 did not contain any protein. Peaks 4, 5, 6 were not analysed.