Over-expression of myrAkt was associated with reduced MAP kinase activation in the <i>An. stephensi</i> midgut.

<p>Midguts from 3–5 day old female HM myrAkt and NTG <i>An. stephensi</i> were dissected at 0.5 h post blood feeding and processed for western blot. Data are represented as the average fold change ± SEM of phospho-protein levels for pERK, p-p38, and p-JNK quantified by densitometry and normalized first to GAPDH to control for protein loading differences and then to phospho-protein levels in NTG controls. Data collected from 6–8 separate cohorts of <i>A. stephensi</i> were analyzed by Student's t-test (alpha = 0.05). <i>P</i> values are noted on the graph.