<p>HeLa cells were transfected with proviral constructs carrying both wt Gag and wt Env (<b>A</b>), wt Gag and Env(ΔCT) (<b>B</b>), Gag carrying the MA mutation and wt Env (<b>C</b>), or comprising both mutated MA and Env(ΔCT) (<b>D</b>), respectively. Fixed cells were stained by indirect immunofluorescence using MAb APR342 and goat anti-mouse Alexa Fluor 532 and MAb 2G12 and goat anti-human Alexa Fluor 647, and subjected to dual-color dSTORM. Scale bar corresponds to 1 µm. An overview of respective cells is presented in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003198#ppat.1003198.s007" target="_blank">Figure S7</a>.</p
<p>(<b>A</b>) Linear representation of Env and the mutants Env ΔV1V2.2, ΔV1V2.4.DNGSEK and ΔV1V2.9.V...
<p>293T cells were transfected with the indicated molecular clones. At 24<a href="http://www.plospat...
<p>Viruses were produced by transfection of HEK293T cells and virions were pelleted from the clarifi...
<p>(<b>A, B</b>) Distribution of HIV-1 Gag and Env at the plasma membrane of HeLa cells transfected ...
<p>(<b>A, B</b>) Distribution of HIV-1 Gag and Env at the plasma membrane of HeLa cells transfected ...
<p>(A) 293T cells were transiently transfected with 3 µg of the indicated plasmid DNA constructs. Fo...
<p>HEK293T cells were transiently co-transfected with SIVmac239 Env mutants and a GFP expression pla...
<p>(<b>A</b>) Western blot analysis of producer cell lysates (<b>Cell</b>) and pelleted viral supern...
<p>Viruses were produced by co-transfection of 293T cells with combinations of Env deficient backbon...
<p>(A) Western blot analysis of XMRV gag expression. HeLa cells were infected with Ad5-XMRV (10 MOI)...
<p>Viruses were produced by transfection of HEK293T cells and virions were pelleted from the clarifi...
<p>(A) Schematic of the EnvB protein showing host range regions hr1and hr2 of SU and the fusion pept...
<p>(A) Schematic representation of REJO WT and eight different SP mutations evaluated in this study....
<p>(A) Jurkat cells were transfected with the indicated molecular clones. At 2-day intervals the cel...
AbstractSIVmac239 and SIVmac1A11 are wild-type viruses encoding Env proteins with full-length or tru...
<p>(<b>A</b>) Linear representation of Env and the mutants Env ΔV1V2.2, ΔV1V2.4.DNGSEK and ΔV1V2.9.V...
<p>293T cells were transfected with the indicated molecular clones. At 24<a href="http://www.plospat...
<p>Viruses were produced by transfection of HEK293T cells and virions were pelleted from the clarifi...
<p>(<b>A, B</b>) Distribution of HIV-1 Gag and Env at the plasma membrane of HeLa cells transfected ...
<p>(<b>A, B</b>) Distribution of HIV-1 Gag and Env at the plasma membrane of HeLa cells transfected ...
<p>(A) 293T cells were transiently transfected with 3 µg of the indicated plasmid DNA constructs. Fo...
<p>HEK293T cells were transiently co-transfected with SIVmac239 Env mutants and a GFP expression pla...
<p>(<b>A</b>) Western blot analysis of producer cell lysates (<b>Cell</b>) and pelleted viral supern...
<p>Viruses were produced by co-transfection of 293T cells with combinations of Env deficient backbon...
<p>(A) Western blot analysis of XMRV gag expression. HeLa cells were infected with Ad5-XMRV (10 MOI)...
<p>Viruses were produced by transfection of HEK293T cells and virions were pelleted from the clarifi...
<p>(A) Schematic of the EnvB protein showing host range regions hr1and hr2 of SU and the fusion pept...
<p>(A) Schematic representation of REJO WT and eight different SP mutations evaluated in this study....
<p>(A) Jurkat cells were transfected with the indicated molecular clones. At 2-day intervals the cel...
AbstractSIVmac239 and SIVmac1A11 are wild-type viruses encoding Env proteins with full-length or tru...
<p>(<b>A</b>) Linear representation of Env and the mutants Env ΔV1V2.2, ΔV1V2.4.DNGSEK and ΔV1V2.9.V...
<p>293T cells were transfected with the indicated molecular clones. At 24<a href="http://www.plospat...
<p>Viruses were produced by transfection of HEK293T cells and virions were pelleted from the clarifi...