Shh and Pdgfrα signals are downregulated in <i>Ftm</i>-negative murine hearts.

<p>(A–D) Real-time PCR analysis of wild-type and <i>Ftm</i>-deficient ventricular (A, B) and atrial tissue (C, D) at E11.5 (A, C) and E14.5 (B, D). (A) Shh target gene expression of <i>Ptc1</i> and Pdgfrα target gene expression of <i>Hif1α</i> are significantly downregulated in E11.5 <i>Ftm</i>^−/− ventricles (n = 3, respectively; <i>Ptc1</i>: p = 0.013; <i>Hif1α</i>: p = 0.005). (B) At E14.5, both signaling pathways are unaffected in <i>Ftm</i>-negative ventricles (n = 6, respectively). (C, D) In <i>Ftm</i>^−/− atria, Shh and Pdgfrα signaling are not significantly altered at E11.5 (n = 6 atria, respectively; C) and at E14.5 (n = 6 atria, respectively; D).