Nicotinic acetylcholine receptor (nAChR) expression on NK cells.

<p>Spleen, or lymph node cell suspensions were obtained RAG2^−/− mice, and FACS was performed as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057495#s2" target="_blank"><i>Materials and Methods</i></a> section. <b>A</b>. Representative dot plots of cell populations before and after sorting are shown in the left- and right-hand panels, respectively. The purity of NK cells reached >98% purity after sorting. <b>B</b>. mRNA was purified from the sorted NK cells, and cDNA was synthesized by reverse transcription. PCR was then performed using primers specific for each mouse nAChR subunit. A positive control (+), using whole mouse brain cDNA, and a negative control (-), without cDNA, were included in each reaction. For each nAChR subunit, bands from the positive control and at least one sample from each cell type were sequenced and confirmed as the correct PCR product. <b>C</b>. nAChR expression assessed by radioligand saturation binding. Data depicted are representative from 2 to 3 separate experiments with similar results.