Susceptibility of break-through colonies to rifampicin and Medihoney.

<p>Break-through colonies growing in the zone of inhibition around the rifampicin disc on TSA plates (clone 1 and clone 2) and TSA plates with 5% sugar solution (clone 3) were selected (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057679#pone-0057679-g002" target="_blank">Fig. 2</a>A). Overnight cultures were prepared in CaMHB and minimum inhibitory concentrations (MIC) of rifampicin (<b>A</b>) and Medihoney (<b>B</b>) were determined. WT is wild type <i>S. aureus</i> NCTC8325.