Identification of possible EDN2 regulated genes using microarrays.

<p>Gene expression was examined using the Mouse 430_2.0 Affymetrix array (4 arrays/genotype) and the 20 most differentially expressed mRNAs in <i>Tg(RHO P347S); Edn2^+/+</i> vs. <i>Edn2^+/+</i> retinas, and in <i>Tg(RHO P347S)</i>; <i>Edn2^−/−</i> vs. <i>Edn2^−/−</i> retinas, at PN21 are shown. All differentially expressed genes in <i>Tg(RHO P347S)</i>; <i>Edn2^+/+</i> retinas showed a significant reduction in expression in <i>Tg(RHO P347S)</i>; <i>Edn2^−/−</i> retinas, with the exception of <i>Nuclear protein 1</i>. The fold reduction is shown to the right of each pair of bars. The detection of <i>Edn2</i> transcripts in <i>Tg(RHO P347S)</i>; <i>Edn2^−/−</i> retinas is due to the expression of partial <i>Edn2</i> mRNAs from the <i>Edn2^−/−</i> locus, in which part of exon 1 and all of exon 2 were replaced with the <i>Neo</i> cassette. n.s. = no statistically significant increase in expression.