Effects of maternal puberty and eCG treatment on intra-oocyte Ca²⁺ reserves of mouse oocytes following in vitro maturation.

<p>The fluorescence intensity value (FIV) for each oocyte was calculated by subtracting the basal intensity value from the peak value. Panels A, B and C show three oocytes with a FIV of 30, 55 and 80, respectively, whereas panel D shows the relative levels of Ca²⁺ in oocytes from prepubertal (Prepb) or adult mice primed with (eCG+) or without eCG (eCG−). Each treatment was repeated three times with each replicate containing at least 20 oocytes. a–c: Values without a common letter above their bars differ (P<0.05).