Hypoxia decreases expression of AQP5 protein and mRNA levels in MLE-12 cells.

<p>(A) Western blot analysis of total protein extract from MLE-12 cells exposed to normoxia and hypoxia (B) Quantitation of the Western blots using β-actin as the loading control (C) Northern blot analysis of total mRNA isolated from cells exposed to normoxia and hypoxia. (D) Quantitation of the Northern blots blot using L32 as the loading control. (E) Western blot analysis of nuclear extracts from cells exposed to normoxia and hypoxia. Lamin B was used as a loading control. Values for the blots are the mean ± SEM (n = 3). N, normoxia. H, hypoxia.