Activations of ERK/NF-κB, JNK/c-Jun, and Akt are induced in arsenite-transformed HELF cells.

<p>Abbreviations: HELF-C, normal HELF cells; HELF-30C, passage-control HELF cells; HELF-30T, arsenite-transformed HELF cells. Densities of bands were quantified by Eagle Eye II software. GAPDH levels, measured in parallel, served as controls. HELF cells were exposed to 0.0 or 1.0 µM arsenite for 30 passages. (A, B) Western blot analyses and (C) relative protein levels (means ± SD, n = 3) of p-ERK, p-JNK, and p-p38 (representative of MAPKs signal pathways); p- NF-κB 65 and p-c-Jun levels (representative transcription factors); and p-Akt level (representative of the PI-3Ks signal pathway). **<i>P</i><0.01 different from HELF-30C cells.