TSA induces transient activation of <i>MAGEA1</i> in melanoma cells.

<p>SK-MEL-23 and EB16-MEL cell lines were exposed to either 300 nM TSA during 24h (<i>A</i>), or 1 µM 5-azadC during 96h (<i>B</i>), and RNA was extracted from the cells at days 1, 4 and 7 after TSA treatment or at days 4, 7, 10 after 5-azadC treatment. <i>MAGEA1</i> expression levels were determined by quantitative RT-PCR. Values, which derive from three independent experiments, were normalized by the <i>ACTINB</i> expression level, and are expressed relative to the levels found in non-treated cells (ctrl). * <i>P</i><0.05, ** <i>P</i><0.01, *** <i>P</i><0.001. <i>C</i>, The effect of TSA and 5-azadC on <i>MAGEA1</i> 5′-region DNA demethylation was assessed by applying MS-PCR to DNA samples extracted 10 days after the beginning of the treatments. Data (fold demethylation) correspond to the relative amount of unmethylated sequences in treated cells reported to that in untreated cells (ctrl). Values represent the mean (± sem) of three independent qMS-PCR experiments. * <i>P</i><0.05, ** <i>P</i><0.01.