CD8⁺ T cell depletion in CBA/J mice.

<p>CBA/J mice were infected with an aerosolized dose of <i>Mtb</i> and from day 90–120 were treated weekly with depletion antibody via intraperitoneal injection then sacrificed at day 125 post-infection. (<b>a</b>) Lungs of anti-CD8⁺ T cell depleted or control mice were homogenized and plated on 7H11 plates and CFU enumerated after 21 days. Absolute number of total CD4⁺ T cells (<b>b</b>) or IFN-γ⁺CD4⁺ T cells (<b>c</b>) after CD8⁺ T cell depletion as determined by flow cytometry. (<b>d</b>) Levels of pulmonary IL-10 after CD8⁺ T cell depletion as determined by ELISA. (<b>e</b>) Lungs of anti-Vβ8 depleted or control mice were homogenized and plated onto 7H11 plates and CFU enumerated. Control group = no treatment and isotype control. Results representative of at least two independent experiments with 5–10 mice per group. Depletion resulted in 95% reduction in cell number, as determined by flow cytometry. * p<0.05, ** p<0.01, *** p<0.001 as obtained by Student's <i>t</i> test.