DNA methylation status of imprinted genes <i>Snrpn</i> and <i>H19.</i>

<p>(A) Bisulfite genomic sequencing was performed to examine the genomic imprinting status of DMRs in the maternal imprinted gene, <i>Snrpn</i>, and the paternal imprinted gene, <i>H19</i>. Open and closed circles represent unmethylated and methylated CpG dinucleotides respectively. Oocytes and granulosa cells were separated from 5-day-old ovarian secondary follicles. Similarly, oocyte-like cells were isolated from clone-G ESC-derived FLSs. Undifferentiated clone-G ESCs were also analyzed as the control. (B) Quantification of DNA methylation levels. The extent of CpG methylation was divided by the total number of CpG sites, based on the result shown in (A).