Binding of CAI peptide and BM2 to WT CA and CA 4Mu Hexamer analyzed by Surface Plasmon Resonance.

<p>(<b>A</b>) Sensorgram data of CAI binding to WT CA. CAI was injected at the highest concentration of 100 µM and ten 3-fold serial dilutions thereof. Each trace was collected in duplicate. (<b>B</b>) Fit of sensorgram data in A by fitting the equilibrium response at each concentration to a simple 1∶1 binding isotherm model. (<b>C</b>) Sensorgram data of CAI binding to hexamer. CAI concentrations used were the same as in A. (<b>D</b>) Attempt to fit sensorgram data in C to a simple 1∶1 binding isotherm model. Due to insufficient response, an accurate equilibrium dissociation constant for this interaction could not be obtained. The K_D for this interaction was simply cited as >100 µM. (<b>E</b>) Sensorgram data for the binding of BM2 to WT CA (black curve, sensorgram: red curve kinetic analysis using a simple 1∶1 kinetic binding model). The K_D for this interaction was calculated from the ratio of the k<i>_on</i> and k<i>_off</i> rate constants obtained from this fit. (<b>F</b>) Sensorgram data for the binding of BM2 to CA 4Mu Hexamer. The affinity of BM2 to hexamer is too weak to allow for quantification of the k<i>_on</i> and k<i>_off</i> rate constants.