<p>Representative images of Western blots of the lysates from stable clones generated with either the pcDNA3.1(+)/<i>MCPH1</i>/myc-His construct or the empty vector pcDNA3.1(+)/myc-His after G418 selection in KB cells. ß–actin was used as a loading control. Abbreviations: KB, parental cell line; V2 and V4, stable clones with the vector only; and, B1, B4, B9 and B10, MCPH1 overexpressing stable clones.</p
<p>(A) Example of a chimeric animal (chimerism of 60 to 70%) that was generated by injection of ES c...
<p>(<b>A</b>) Schematic representation of the control Ires–Luc and Mini5B–Luc vectors. The insert en...
(A) MEF-Ld cells expressing vector control or F-MC80L were immunoprecipitated with anti-Ld followed ...
Mutations in the human gene MCPH1 cause primary microcephaly associated with a unique cellular pheno...
<p>Western blot analysis of K8/18 using mAbs to K8 and K18 respectively (<b>A</b>) MDA MB 435 K8 ove...
<p>(A) Cells were transduced with GFP-tagged coding sequence of full-length MCPH1 cDNA in a conditio...
<p>(A) Genomic DNA of ES cell clones 1, 14, 18 and 19 and of wildtype ES cells (wt Bruce 4) was dige...
Figure S1. Knock-out strategy for the MycΔ allele. (A) Targeting strategy is shown along with the ge...
<p>(A) Western blots demonstrating that <b>MUC1 protein</b> is lower in both cell lysates (upper lef...
Mutations in the human gene MCPH1 cause primary microcephaly associated with a unique cellular pheno...
<p>(A) Bright field images of the selected clones and parental cells. (B) Western blots performed on...
<p>(A) HeLa cells were arrested in G1 phase by double thymidine block. Cultures harvested at various...
<p>Western blot analysis of K7 using mAb to K7 (<b>A</b>) MDA MB 435 K8 over-expressed (K8C1, C2 and...
One of the major hurdles of isolating stable, inducible or constitutive high-level producer cell lin...
One of the major hurdles of isolating stable, inducible or constitutive high-level producer cell lin...
<p>(A) Example of a chimeric animal (chimerism of 60 to 70%) that was generated by injection of ES c...
<p>(<b>A</b>) Schematic representation of the control Ires–Luc and Mini5B–Luc vectors. The insert en...
(A) MEF-Ld cells expressing vector control or F-MC80L were immunoprecipitated with anti-Ld followed ...
Mutations in the human gene MCPH1 cause primary microcephaly associated with a unique cellular pheno...
<p>Western blot analysis of K8/18 using mAbs to K8 and K18 respectively (<b>A</b>) MDA MB 435 K8 ove...
<p>(A) Cells were transduced with GFP-tagged coding sequence of full-length MCPH1 cDNA in a conditio...
<p>(A) Genomic DNA of ES cell clones 1, 14, 18 and 19 and of wildtype ES cells (wt Bruce 4) was dige...
Figure S1. Knock-out strategy for the MycΔ allele. (A) Targeting strategy is shown along with the ge...
<p>(A) Western blots demonstrating that <b>MUC1 protein</b> is lower in both cell lysates (upper lef...
Mutations in the human gene MCPH1 cause primary microcephaly associated with a unique cellular pheno...
<p>(A) Bright field images of the selected clones and parental cells. (B) Western blots performed on...
<p>(A) HeLa cells were arrested in G1 phase by double thymidine block. Cultures harvested at various...
<p>Western blot analysis of K7 using mAb to K7 (<b>A</b>) MDA MB 435 K8 over-expressed (K8C1, C2 and...
One of the major hurdles of isolating stable, inducible or constitutive high-level producer cell lin...
One of the major hurdles of isolating stable, inducible or constitutive high-level producer cell lin...
<p>(A) Example of a chimeric animal (chimerism of 60 to 70%) that was generated by injection of ES c...
<p>(<b>A</b>) Schematic representation of the control Ires–Luc and Mini5B–Luc vectors. The insert en...
(A) MEF-Ld cells expressing vector control or F-MC80L were immunoprecipitated with anti-Ld followed ...