Tumor suppressor activity of MCPH1.

<p>(A) The analysis of cell proliferation in KB (parental cell line), empty vector stable clones (V2 and V4) and MCPH1 overexpressing stable clones (B1 and B9) by the BrdU assay. The graph represents the OD (optical density) measurment at 450 nm. Note the B1 and B9 clones incorporated significantly lesser BrdU than the V2, V4 and KB cells. The values are the mean±SD of three independent experiments. Abbreviations: ns, no statistical significance; and, *** indicates p<0.001. (B) Anchorage-independent growth of KB, V2, V4, B1 and B9 cells in soft agar. Note MCPH1 overexpression reduces colony growth of B1 and B9 clones as compared to KB, V2 and V4 cells. (C) The <i>in vivo</i> tumorigenic potential of V2, V4, B1 and B9 cells in nude mice. The representative images of the nude mice with tumors (upper panel) and the harvested tumors (lower panel) are shown. Note that the overexpression of MCPH1 reduces tumor growth in nude mice. The arrows mark the tumors. (D) Tumor volumes at different time points. The values given are the mean±SD from five animals. * indicates p<0.05.