miR-27a targets both the seed regions of <i>MCPH1</i> cloned separately.

<p><b>(</b>A) The luciferase reporter assay of different constructs harboring seed region 1 (SDR1) of <i>MCPH1</i> 3′-UTR in KB cells. Note a significantly reduced luciferase activity in cells co-transfected with pMIR-Report-3′-UTR-S1 and pcDNA3/pre-miR-27a/<i>EGFP</i> (0.2 or 0.4 µg) in comparison to those with pMIR-Report, suggesting miR-27a targets SDR1 of <i>MCPH1</i>. As expected, no significant difference in luciferase activity was observed in cells co-transfected with pMIR-Report-3′-UTR-AS1 or pMIR-Report-3′-UTR-M1 with pcDNA3/pre-miR-27a/<i>EGFP</i> (0.2 or 0.4 µg) in comparison to those with pMIR-Report. (B) The luciferase reporter assay of different constructs harboring seed region 2 (SDR2) of <i>MCPH1</i> 3′-UTR in KB cells. As with SDR1, miR-27a also targets SDR2.