Effect of LPIAT1 knockout on brain phosphoinositide lipid molecular species.

<p>Brains from 13 day old littermates expressing (LPIAT1^+/+ (WT)) or lacking (LPIAT1^−/− (KO)) LPIAT1 were homogenised and lipid extracted from 0.5 mg wet weight as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058425#s2" target="_blank">Materials and Methods</a>. Targeted molecular species of PtdIns (<b>A</b>), PtdInsP (<b>B</b>), PtdInsP₂ (<b>C</b>) and PtdInsP₃ (<b>D</b>) were detected by MRM mass spectrometric analysis as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058425#s2" target="_blank">Materials and Methods</a>. Data are expressed as moles/mg protein, normalized to relevant internal standards. Shown are mean ± SD, n = 4 for both WT and KO. Data were analyzed by T-test. *p≤0.05, **p≤0.005.