Multivariate analysis of CUR dose-related metabolite data.

<p>A- PLS-DA of metabolite data obtained in MCF7 and MDA-MB-231 cells types responding to 10 mg.l⁻¹ CUR. Comapping of metabolites and 3 factors (cell type: MCF7 and MDA-MB-231) and common response to CUR at 10 mg.l⁻¹ (CUR10). Metabolite abbreviations, as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057971#pone-0057971-t001" target="_blank">Table 1</a>. B- PLS-DA of metabolite data obtained in MCF7 and MDA-MB-231 cells types responding to 50 mg.l⁻¹ CUR. Comapping of metabolites and 3 factors (cell type: MCF7 and MDA-MB-231) and common response to CUR at 50 mg.l⁻¹ (CUR50). Metabolite abbreviations, as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057971#pone-0057971-t001" target="_blank">Table 1</a>. C- PCA of CUR dose related data. Each score corresponds to an individual measurement and is labeled CTL or the corresponding CUR dose level. F1 and F2, first 2 principal components with the percentage of total variance of data they account for. Two elliptic clouds separate, to the left, the set of CTL and low dose CUR (0.5, 2.5, and 10 mg.l⁻¹) samples, and, to the right, the set of high dose CUR (25 and 50 mg.l⁻¹) samples. Grey arrow, trajectory between low and high dose clouds. D- Loading plot of PCA. Metabolite abbreviations, as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057971#pone-0057971-t001" target="_blank">Table 1</a>. E- Partial correlation of CUR dose-related data with available covariables (DNA content, tail DNA and GST activity). Red, positive correlation; green, negative correlation; left, color scale of correlations, between -1 and +1. F- PLS-DA of metabolite data, after fitting to a hormetic model, showing the VIP plot of the 5 most discriminant metabolites with hormetic behaviour (left). Corresponding loading plot (right). Metabolite abbreviations, as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057971#pone-0057971-t001" target="_blank">Table 1</a>.