Duration of exposure-related response to CUR.

<p>Cells were treated with 10 mg.l⁻¹ CUR and followed for 96 h. A- Cellular DNA content in attached MCF7 tumor cells relative to time-matched CTL. DNA content was measured using the Hoechst fluorescence intensity assay. *, P<0.05, **, P<0.01 CUR vs. CTL, Mann-Whitney test. B- Tail DNA relative to time-matched CTL. DNA. Tail DNA was assessed using the alkaline Comet assay. *, P<0.05, CUR vs. CTL, Mann-Whitney test. C- Total GST activity relative to time-matched CTL. GST activity was measured using the Cayman Chemical Company assay kit. **, P<0.01, CUR vs. CTL, Mann-Whitney test. D- Metabolite variations relative to time-matched CTL. #, P<0.05; *, P<0.025; $, P<0.01, CUR <i>vs.</i> CTL, Mann-Whitney test. E- PCA of duration of exposure-related data. The scores plot is displayed with individual scores labeled by their duration of exposure, between 24 h and 96 h, with CTL data also displayed. F1 and F2, first 2 principal components with the percentage of total variance of data they account for. Grey arrows, duration of exposure-related trajectory. F- Corresponding loading plot of PCA of duration of exposure-related data. Metabolite abbreviations, as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057971#pone-0057971-t001" target="_blank">Table 1</a>.