Origin of samples collected on tomato plants co-infected with TYX and TOX.

<p>Samples were collected from four independent experiments as indicated. Tomato plants were co-infected with <i>Tomato yellow leaf curl virus</i> (TYX) and <i>Tomato leaf curl Comoros viru</i>s;(TOX) either by agro-inoculation (blue lines) or by vector-inoculation (red lines). The vertical dotted red lines represent the vector transmission in which plant 1 and 4 were used as source plants. In Experiments 1–3, the percentage of recombinant genomes and the number of genomes analyzed (between brackets) were indicated at each sampling time. When the same recombinant genome was isolated from at least two distinct plants it is indicated by a symbol (▪, ▴, □) or by its name, (R10). (-) indicates no data obtained. In Experiment 4, the frequency of viral DNA of TYX and recombinant genomes (REC) in the viral population were estimated at 18 and 30 days post inoculation (dpi) by real time PCR. The frequency was estimated from the five plants which were detected positive for recombinants at 18 dpi and from the 22 plants detected positive at 30 dpi.