Effects of AND on angiogenesis and on the phosphorylation of signaling proteins in MPM xenografts.

<p>(A) Left panels. Detection of angiogenesis factors on membrane antibody arrays by chemiluminescence (one representative experiment is shown). Images are obtained with a CCD camera after 60-s exposures using a Quantity One Imaging system. Each factor is represented by duplicate spots. (A) Right panel. Net light intensity for TIMP-1 and TIMP-2, detected on the basis of gray-scale levels using Quantity One software. Data are means±SD of measurements carried out on 2 membranes with 2 spots each. * = p<0.01 according to t test. (B) Phosphorylation status of different cell growth-related proteins evaluated by the Bio-PlexTM multiplex system (see Methods). Fluorescence measurements carried out on 4 different samples are plotted. * = p<0.01 according to t test.