Expression and phenotypes of the riboflavin transporters.

<p>(A) Quantitative RT-PCR analysis of <i>rft-1</i> expression. The data were normalized relative to the housekeeping gene β-actin (<i>act-2)</i>. All worms were raised on HT115(DE3) bacteria harboring either the <i>rft-1</i> RNAi feeding plasmid (<i>rft-1</i> RNAi worms) or the empty vector, pPD129.36 (all other worms). Additionally, some worms were raised on plates supplemented with riboflavin. (B) The effects of RNAi on egg laying. Here, worms were reared on bacteria harboring the RNAi empty vector (Controls), the <i>rft-1</i> RNAi feeding vector, the <i>rft-2</i> RNAi feeding vector, or both RNAi feeding vectors. (C) Micrographs of a worm expressing the transcriptional reporters P<i>rft-1::gfp</i> and P<i>rft-2::mCherry</i>. Both reporters were expressed in the intestine, whereas only mCherry was found in the pharynx, and only GFP was found in neuronal support cells that spanned the length of the body.