Targeting of HDAC4 in IGROV-1 cells by hsa-miR-302b.

<p>IGROV-1 cells were transfected with 50 nmol/l hsa-miR-302b or a scrambled oligonucleotide and RNA and proteins were collected after 72 h. HDAC4 mRNA levels were evaluated by RT-qPCR (A) and protein expression was evaluated by Western blot (B). GAPDH was used to normalize protein loading per lane. Data are representative of 6 independent experiments with superimposable results. (C) Schematic representation of the interaction between hsa-miR-302b and the binding site on the wild-type HDAC4-3′UTR and the mutated control. (D) Relative luciferase activity in IGROV-1 cells for HDAC4-3′UTR-wt co-transfected with reporter vector and with hsa-miR-302b precursor molecule or negative scrambled control for 48 h. (E) Relative luciferase activity in IGROV-1 cells for HDAC4-3′UTR-mut co-transfected with reporter vector and with hsa-miR-302b precursor molecule or negative scrambled control for 48 h.