The liver in the Fgl1 knockout mouse.

<p>A) Fgl1 transcript is absent in the livers of the knockout mouse pre and post PH. Note the expected induction of Fgl1 in the wild type mouse after PH (P<0.01 for Fgl1 at baseline and 48 h after PH, n = 3 per group) B) Fgl1 protein is absent in the livers of the knockout mouse before and after PH while it is detectable at baseline and induced after PH in the wild type mouse. C) <i>Fgl1^−/−</i> mice are larger than wild type mates as early as three weeks after birth (P<0.0001, n = 5 for each group). D) Representative graph of change in weight over time for <i>Fgl1^+/+</i> (n = 8 for first 4 weeks, n = 4 for last three weeks) and <i>Fgl1^−/−</i> (n = 9 for first 4 weeks and n = 8 for last three weeks). E) Liver mass is not different between <i>Fgl1^+/+</i> and <i>Fgl1^−/−</i> mice (n = 9 for each group). F) Liver weight to body weight ratio is smaller for <i>Fgl1^−/−</i> mice (P = 0.008, n = 9). G) Marked lipid accumulation in the livers of <i>Fgl1^−/−</i> mice after PH. Top: gross images of representative livers from <i>Fgl1^+/+</i> (left) and <i>Fgl1^−/−</i> (right). Arrows indicate remnant liver lobes. Middle: H&E images at 40× magnification of liver sections from <i>Fgl1^+/+</i> and <i>Fgl1^−/−</i> mice at 48 h post PH and bottom: similar H&E images at 96 h post PH. Note the resolution of steatosis in the <i>Fgl1^−/−</i> mouse by 96 h after PH. H) Triglyceride (TG) content of liver extracts from <i>Fgl1^+/+</i> and <i>Fgl1^−/−</i> mice before and after PH. The difference between <i>Fgl1^−/−</i> and <i>Fgl1^+/+</i> at 48 h after PH is significant (P = 0.014, n = 3–5 per cohort for experiments). I) mRNA levels of lipid regulatory genes at 48 h after PH (P = 0.011, 0.014 and 0.037 respectively for PPARα, PPARδ and FATP5 but is otherwise non significant). n = 4 for <i>Fgl1^+/+</i> except FATP5 where n = 3 per group. n = 5 for <i>Fgl1^−/−</i>.