Viability of yeast cells producing S100A8 and S100A9 on the background of a <i>ts</i> mutation in an essential gene.

<p>(A) wild type and <i>cdc53-1</i> ts mutant cells expressing p<i>YES2-S100A8</i> or p<i>YES2-S100A9</i> were spotted on glucose or galactose plates and photographed after 72 h. (B<b>)</b> Semi-denaturing agarose gel. After 2 days of induction ^GFPS100A9 forms aggregates in wild type and ts strain <i>cdc53-1</i> at 30°C and 32°C. Total cell extracts (180 µg) were resolved using SDD-AGE. (C<b>)</b> Ten-fold dilutions of <i>cdc53-1</i>, <i>cdc34-2</i>, <i>srp1-31,</i> and <i>sec27-1</i> yeast cells transformed with p<i>TET-S100A8</i> or p<i>TET-S100A9</i> were spotted on SD plates with (inducing) or without (non-inducing) 5 µg/ml doxycycline and photographed after 72 h.