siRNAs targeting ITPR2.

<p>(A) The relative expression levels of the target gene: ITPR2. Two good siRNAs (-G-) and two bad siRNAs (-B-) are designed to target the ITPR2 gene. Most of them can reduce the target gene expression level to below 50%. (B–F) The relative expression levels of off-target genes predicted by Picky. Different subfigures correspond to different off-target genes that are associated with different siRNAs respectively. All expression levels are calibrated to the negative transfection controls (Neg: dark grey bars). The light grey bars correspond to treatments with siRNAs except the one with off-target effect predicted by Picky. A test was performed for significant reduction of gene expression by the siRNA with predicted off-target effect compared with other siRNAs. If p-value <0.05, the treatment using siRNA with the predicted off-target effect is red and labelled by *. Otherwise, it is pink. Remarkably, the expression level of UBE2R2 was reduced by 60% by ITPR2-B-design.