Confirmation of gene-expression patterns up or downregulated during melon MFA.

<p>qRT-PCR analysis of 47 selected genes in various melon tissues at 36, 38 and 40 DPA: AZ (-Δ-), pedicel (proximal non-AZ, -□-) and fruit mesocarp (distal non-AZ, -Ο-). Analysis of transcript levels of genes by quantitative RT-PCR. Genes and their primers are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058363#pone.0058363.s027" target="_blank">Table S16</a>. Relative expression values were normalized to the lowest expression value taken as 1. The data represent the mean values (±SEs) of duplicate experiments from three independent biological samples. Broken lines (–▴–) show expression profiling of genes in the melon AZ as reconstructed from the 454 pyrosequencing transcriptome. Broken line indicates the total read count in RPKMx1000 for each gene after normalization across the samples: AZ at 36, 38 and 40 DPA.