IFNAR mRNA analysis in undifferentiated BE(2)-C and differentiated BE(2)-C/m human neuronal cells.

<p>(A) Semi-quantitative RT-PCR analysis of total NGFR, IFNAR1, and IFNAR2 mRNA transcript expression. Ribosomal RNA (rRNA) was analyzed as a loading control and ten-fold serial dilutions of cDNA were used for PCR. (B) Quantitative RT-PCR analysis of NGFR, IFNAR1, and IFNAR2 mRNA transcript expression. Results are presented as inverse rRNA-normalized threshold cycle (1/ΔC_t) values so that increased mRNA levels are associated with increased bar height. n.s., not significant; *<i>p</i><0.05. (C) Schematic of IFNAR2 total (white and black arrows) and isoform-specific (white and grey arrows) primer design. (D) Semi-quantitative RT-PCR analysis of isoform-specific IFNAR2 mRNA transcript expression. Ten-fold serial dilutions of cDNA from the indicated cell line were used for PCR. Representative results from one of four independent experiments are shown.