miR-191/425 expression is associated with ERα.

<p>(A) Expression of miR-191, miR-425 and DALRD3 mRNA in 44 breast tumor specimens with different status of estrogen receptor α (20 ER− and 24 ER+) (p-value<0.001). (B) In-situ hybridization for miR-191 and miR-425 and scrambled control probe (SCR) on breast cancer tissue array containing 132 cores of breast cancer tissues. Bars are 200 µm and inserts represent higher magnification (20×) of selected areas. (C) Schematic representation of miR-191 and miR-425 location in the first intron of the putative protein coding gene DALRD3 with its two different isoforms. (D) Heat map representing the status of ERα, progesterone receptor (PR) and HER2, confirmed by western blot analyses, in a set of 15 breast cancer cell lines (Yellow: positive; Blue: negative). Northern blot analyses depicting miR-191 and miR-425 expression levels. snRNAU6 levels were used as a loading control. Expression levels of all isoforms of DALRD3 mRNA were determined by Taqman qRT-PCR. All error bars represent mean s.d.