Influence of NCS23766 and Y27632 on the activity of Rac1 and ROCK on tissue culture plastic.

<p>Studies were run for 1 week and 3 days to quantify the temporal suppression of the inhibitors. NCS23766 significantly reduced Rac1 activity and Y27632 inhibited the activity of ROCK. Alizarin red staining of wells demonstrated increased mineralization in the presence of ROCK inhibitors. Treatments were analyzed via one-way ANOVA with a Bonferroni post-test (* denotes significance of p<0.05 between treatments).