Influence of topography, Rac1 and ROCK inhibition on expression of (a) BMP-2, (b) osteocalcin and (c) bone sialoprotein in osteoblasts at 24 h post seeding.

<p>ALP mRNA levels were significantly increased on PT by both inhibitors, but only ROCK had an effect on SLA. No significant differences were observed in BMP-2 or BSP levels under any treatment, but ROCK inhibition significantly increased osteocalcin levels on both PT and SLA. Graphs represent mean ± SEM of 3 independent experiments completed with 4 replicates per experiment. Treatments were analyzed via two-way ANOVA with a Bonferroni post-test (# denotes significance of p<0.05 between PT and SLA, * denotes significance of p<0.05 between treatments within a surface type).