Influence of topography, Rac1 and ROCK inhibition on expression of (a) BMP-2, (b) osteocalcin and (c) bone sialoprotein in osteoblasts at 7 days post seeding. ROCK inhibition significantly increased osteocalcin and BSP mRNA levels on both PT and SLA compared to control surfaces.

<p>Graphs represent mean ± SEM of 3 independent experiments completed with 4 replicates per experiment. Treatments were analyzed via two-way ANOVA with a Bonferroni post-test (# denotes significance of p<0.05 between PT and SLA, * denotes significance of p<0.05 between treatments within a surface type).