Analysis of <i>OsFIE2</i>-RNAi plants.

<p>(A) Quantitative real-time PCR analysis of <i>OsFIE2</i> gene expression in different RNAi lines and wild type. Rice Glyceraldehyde 3-phosphate dehydrogenase (<i>GAPDH</i>) was used as internal control. Same amount of cDNA template was used in each sample. Primers are listed in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003322#pgen.1003322.s006" target="_blank">Table S3</a>. (B) Comparison of mature panicles of <i>OsFIE2</i>-RNAi line NO3 (bottom) and wild type (top). Panicles of RNAi plants contain more partially filled seeds and less filled seeds when compared to wild type panicles. Partially filled or unfilled seeds are marked with arrow in panicle of OsFIE2-RNAi plant. (C) Images of pollen viability tests. Pollen grains are stained with I₂-KI solution. Viable pollen grains are stained dark and nonviable pollen grains are stained light yellow. (i) Image of wild-type pollen. (ii) Image of <i>OsFIE2</i>-RNAi line NO3 pollen. The scale bars = 2 cm in (B) and 100 µm in (C).