Reverse Transcription–PCR and ChIP PCR analysis of helix-loop-helix DNA binding domain containing gene in wild-type and <i>OsFIE2</i>-RNAi plants.

<p>(A) Reverse Transcription-PCR analysis of the expression of helix-loop-helix DNA binding domain containing gene in endosperm tissue. GAPDH was used as internal control. Same amount of cDNA template was used in each sample. Primers are listed in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003322#pgen.1003322.s006" target="_blank">Table S3</a>. (B) ChIP PCR analysis of helix-loop-helix DNA binding domain containing gene in wild type and <i>OsFIE2</i>-RNAi plants. Chromatin is isolated from endosperm tissue of wild type and <i>OsFIE2</i>-RNAi plants. Antibodies for H3K27me3 were used for chromatin immunoprecipitation (ChIP). Input: DNA sample extracted from the chromatin before ChIP enrichment. ChIP: DNA sample extracted from H3K27me3 ChIP enriched chromatin. Mock: DNA sample that went through the immunoprecipitation procedure without antibody.