SUN-1 S12 phosphorylation in male meiosis.

<p>(A) Representative TZ nuclei of a <i>sun-1(wt)</i> hermaphrodite gonad (upper panel) and a <i>sun-1(wt)</i> male gonad (lower panel) to highlight SUN-1 (anti-GFP, green), anti-Him-8 (red), and DAPI (blue). (B) Representative nuclei from wild-type hermaphrodite TZ (upper panel) and wild-type male TZ (lower panel) stained with anti-SUN-1 S12Pi (green), anti-HIM-8 (red), and DAPI (blue). (B′) Representative nuclei from <i>him-3 (gk149)</i> hermaphrodite TZ (upper panel) and <i>him-3 (gk149)</i> male TZ (lower panel) stained with anti-SUN-1 S12 Pi (green), anti-HIM-8 (red), and DAPI (blue). (B″) Representative nuclei from <i>sun-1(jf18)</i> hermaphrodite TZ (upper panel) and <i>sun-1(jf18)</i> male TZ (lower panel) stained with anti-SUN-1 S12 Pi (green), anti-HIM-8 (red), and DAPI (blue). (C) Representative wild-type TZ nuclei of a hermaphrodite gonad (upper panel) and a male gonad (lower panel) costained with anti-PLK-2 (green), anti-Him-8 (red), and DAPI (blue). (D) Relative time of residency in different meiotic stages in different <i>sun-1</i> phosphorylation mutant backgrounds in males, assessed by presence of SUN-1 aggregates; zones were normalized to the individual length of the scored gonad (from meiotic entry to diplotene). Nuclei were sorted into two categories: “TZ” (dark green, more than one SUN-1 aggregate) and “pachytene” (red, no SUN-1 aggregates). (E) Pachytene zone nuclei of <i>sun-1(wt)</i> and <i>sun-1(6E)</i> stained with anti-HIM-8 (blue), anti-SYP-1 (yellow), and DAPI (red). Note that the single male X chromosome was unsynapsed in both WT and <i>6xE</i> substitution lines.