Effect of reverse transcription and proteasomes in restricting cells on large N-MLV subviral complexes.

<p>PgsA-huTRIM5α (huT5α) and control pgsA cells (none) were infected with VSV-G pseudotyped N-MLV (IN-3×HA) in the presence of either 1 mM AZT alone or 1 mM AZT together with 2 µM MG132 for 2 hours. Samples were processed as explained in legend to <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003214#ppat-1003214-g001" target="_blank">Fig. 1</a> and in Materials & Methods. (A) Protein samples in sucrose fractions were analyzed by western blotting using antibodies against CA (p30) and HA tag for detection of IN. (B, C) Viral RNA in parallel fractions of AZT treated (B) or AZT and MG132-treated (C) samples were reverse-transcribed and analyzed by Q-PCR. Data is from a representative experiment.