Transcriptional profiling of opaque filamentation.

<p><i>MTL</i><b>a</b> white (RBY717) or opaque (CAY2903) strains were incubated in SCD medium, a non-filament inducing condition, or sorbitol (SOR) and low phosphate (LP) media, conditions that specifically induce filamentation of opaque cells but not white cells. Cells were harvested after 12 hours growth (LP medium) or 16 hours growth (SOR medium) and cDNA prepared and hybridized against custom <i>C. albicans</i> ORF microarrays. (A) Comparative expression of opaque cell filamentation genes and white hyphal genes. Columns 1 and 2 represent opaque filamentation genes in LP and SOR media (relative to SCD controls) that passed SAM filters. Columns 3 and 4 represent hyphal-induced genes in white cells (serum-treated cells at 1 or 2 h compared to untreated cells) using data from Kadosh and Johnson <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003210#ppat.1003210-Kadosh1" target="_blank">[36]</a>. Top panel; genes induced in both opaque and white filaments. Bottom panel, genes induced in opaque filaments but not white filaments. Grey boxes indicate no data available. (B) Genes expressed in white hyphal cells but not expressed in filamenting opaque cells. Top panel; genes induced in white hyphal cells only. Bottom panel, genes induced in white hyphal cells and either in opaque LP filaments or SOR filaments (but not both). op, opaque cells; wh, white cells. Gene numbers refer to orf19 gene identifiers (e.g. 1774 refers to orf19.1774).