PTMi spot identification and characterisation.

<p>(<b>A</b>) 2D density plot of local STYK AA density windows plotted against local PTM density with a histogram showing the number of local peaks. The 500 most outlying data points are plotted as points on the density plot, however with substantial overlay, preventing visualization of all data point. Some PTM regions contain a higher PTM density than modifiable residues, highlighting regions which are key candidates for direct ubiquitination:acetylation competition on the same lysine residues. Specific proteins with densely modified regions are indicated through coloured circles. (<b>B</b>) 2D density plot of the number of 20 AA windows containing more than 1 PTM. (<b>C</b>) The number of high density windows that contain multiple PTMs compared to 100 random annotation permutation simulations. (<b>D</b>) Overlap analysis between individual PTM 20AA windows and annotated protein domains. Int: 20AA window internal to a protein domain, Lg: Large overlap with a proteins domain (>10AAs), Sm: Small overlap with a protein domain, Ext: 20AA window external to an annotated protein domain. (<b>E</b>) Overlap analysis between individual PTM 20AA windows and predicted protein disorder. High: Every amino acid is predicted to be disordered, Med: 11–19 AAs in a window are predicted to be disordered, Low: 1–10 AAs in a window are predicted to be disordered. (<b>F</b>) Frequency of 20AA windows across a protein sequence that are mutated in cancerous cells, sorted based on their protein domain annotation (coloured bars). The frequency of 20AA windows outside of annotated protein domains that are mutated in cancerous cells, sorted based on their PTM density (Grey bars).